Roberto De Filippo

Neuroscience PhD

About Me

I am a neuroscientist interested in the effect of neuromodulators on sensory perception. Currently my main focus is serotonin, in vivo recordings with high-density probes (Neuropixel) and neural decoding. I have experience using: Python (pandas, multiprocessing, xarray, numpy, HoloViz), Matlab, Tensorflow, Max/MSP, Pure Data, Hugging Faceand git.

Experience

Allen Institute for Neural Dynamics, Seattle

Visiting scientist

November 2024

https://alleninstitute.org/division/neural-dynamics/

Focusing on the effect of psilocybin on neuronal activity

Ott lab, Humboldt-Universität zu Berlin

Postdoc

2024-Now

https://torbenottlab.org/

Psychedelics, large scale recordings, predictive coding in rodents. In collaboration with the Allen Institute via Openscope.

Schmitz lab, Charité-Universitätsmedizin Berlin

Postdoc

2023-2024

https://schmitz.neurocure.de/

Analysis of large-scale open access datasets.

Schmitz lab, Charité-Universitätsmedizin Berlin

Postdoc

2021-2022

https://schmitz.neurocure.de/

Analysing large open-access datasets, focusing on hippocampal ripples propagation.

Schmitz lab, Charité-Universitätsmedizin Berlin

PhD student

January 2016 - 2021

https://schmitz.neurocure.de/

My PhD project is focused on serotonergic modulation of slow oscillations in entorhinal cortex, it led to the publication “Somatostatin interneurons activated by 5-HT2A receptor suppress slow oscillations in medial entorhinal cortex (Elife, 2021)”. We identified a novel mechanism by which serotonin is able to alter cortical network activity. During the course of this project I employed the following techniques: in vitro patch clamp, silicone probe recording in anesthetized mice, optogenetics, pharmacology, immunohistochemistry and confocal imaging. I also became proficient with Matlab and Python for data analysis.

Poulet lab, Max Delbrück Center (MDC)

PhD student

February 2015 - December 2015

https://www.mdc-berlin.de/poulet

I worked under the supervision of Nevena Milenkovic investigating temperature perception in mice, a follow-up to her work “A somatosensory circuit for cooling perception in mice (Nature Neuroscience, 2014)”. I have performed in vivo whole cell patch clamp recording from anesthetized mice, thalamic drug injections and behavioral training.

Brecht lab, Bernstein Center for Computational Neuroscience (BCCN)

Lab rotation

October 2014 - January 2015

https://www.activetouch.de/

During my lab rotation in the Brecht lab I assisted Dr. Shimpei Ishiyama in his project later published as “Neural correlates of ticklishness in the rat somatosensory cortex (Science, 2016)”. The aim of his project was to understand the neurophysiological basis of ticklishness. My work was to assess if rats, like humans, have body areas more susceptible to tickling compared to others. A Matlab-based system was used to record 50-kHz USVs (ultra-sonic vocalization). USVs emission rate was used as a measure of ticklishness. In addition to this psychophysics experiment I assisted to in vivo tetrodes recording and electrical microstimulation in S1.

Cherubini lab, International School for Advanced Studies (SISSA).

Master student

July 2012 - October 2013

https://www.sissa.it/

I did my internship in Professor Enrico Cherubini’s lab working as an in vitro electrophysiologist. My thesis focused on the cis-trans isomerase Pin1 role in excitatory synaptic signaling. I recorded, using the whole-cell configuration of the patch clamp technique, CA1 pyramidal cells in mouse brain slices while stimulating the Schaffer collaterals and applying selective blockers of GABAA, AMPA and NMDA receptors. I performed post-hoc morphological reconstruction on a subset of neurons using biocytin and confocal microscopy. My experiments contributed to the publication of “Pin1 Modulates the Synaptic Content of NMDA Receptors via Prolyl-Isomerization of PSD-95 (Journal of Neuroscience, 2016)”

Education

Charité – Universitätsmedizin Berlin

Doctor of Philosophy in Medical Neurosciences (PhD)

summa cum laude

University of Trieste

Master's degree in Neuroscience

110/110 cum laude

University of Milan

Bachelor's degree in Biology

103/110

Publications

Transcriptomic mapping of the 5-HT receptor landscape (online app)
Patterns 2024 Aug 29. doi: 10.1016/j.patter.2024.101048.
De Filippo R*, Schmitz D
* = Lead contact & corresponding author
Synthetic surprise as the foundation of the psychedelic experience
Neuroscience & Biobehavioral Reviews 2024 Jan 12. doi: 10.1016/j.neubiorev.2024.105538. PMID: 38220035.
De Filippo R*, Schmitz D
* = corresponding author
Differential ripple propagation along the hippocampal longitudinal axis
Elife 2023 Apr 13. doi: 10.7554/eLife.85488. PMID: 37052307.
De Filippo R*, Schmitz D*
* = corresponding authors
Somatostatin interneurons activated by 5-HT2A receptor suppress slow oscillations in medial entorhinal cortex
Elife 2021 Mar 31. doi: 10.7554/eLife.66960. PMID: 33789079.
De Filippo R*, Rost BR, Stumpf A, Cooper C, Tukker JJ, Harms C, Beed P, Schmitz D
* = corresponding authors
Layer 3 Pyramidal Cells in the Medial Entorhinal Cortex Orchestrate Up-Down States and Entrain the Deep Layers Differentially
Cell Reports 2020 Dec 8. doi: 10.1016/j.celrep.2020.108470. PMID: 33296656.
Beed P*, De Filippo R*, Holman C*, Johenning FW, Leibold C, Caputi A, Monyer H, Schmitz D
* = equal contribution
Pin1 Modulates the Synaptic Content of NMDA Receptors via Prolyl-Isomerization of PSD-95
Journal of Neuroscience 2016 May 18. doi: 10.1523/JNEUROSCI.3124-15.2016. PMID: 27194325; PMCID: PMC6601765.
Antonelli R, De Filippo R, Middei S, Stancheva S, Pastore B, Ammassari-Teule M, Barberis A, Cherubini E, Zacchi P.

Conferences

  • 2024 CSHL Neuronal Circuits, Cold Spring Harbor. Poster ‘Transcriptomic Mapping of the 5-HT Receptor Landscape’
  • 2024 COSYNE, Lisbon.
  • 2018 FENS, Berlin. Poster ‘Serotonergic modulation of slow oscillations’
  • 2019 INSIGHT, Berlin. Poster ‘Serotonergic modulation of slow oscillations’
  • 2018 FENS, Berlin. Poster ‘Serotonergic modulation of slow oscillations’

Awards

  • 2024 Allen Institute Openscope
  • 2015 Neurocure fellowship

Various interests